Hindering the biological crosstalk of autophagy and mitochondrial function underlying pancreatic ductal adenocarcinoma

19459001 crosstalk of autophagy and mitochondrial function underlying pancreatic ductal adenocarcinoma” title=”Ectopic SDHB saves the mitochondrial dysfunction upon autophagy inhibition in PDAC. PDAC cells revealing SDHB and control vector were treated with CQ followed by immunoblotting for this proteins (A) and evaluated for (B) OCR (combined information from n = 2 experiments). (C) Representative TEM pictures of cells are displayed in scenario comparable to (A) followed by mitochondrial crista length (D; n = 18 special cristae for all groups) and lumen width (E; n = 18 special cristae for all groups) decision. Red arrows in (C) suggest the normal inefficient mitochondria. ATG5 was reduced in SDHB overexpression and control PDAC cells for immunoblotting of indicated proteins (F). Under conditions comparable to (F), cell expansion (G; integrated information of n = 5 experiment) and OCR was identified (H; integrated information of n = 2 experiments). Information are implies ± SD, and P worths were measured utilizing one-way ANOVA with Tukey’s post hoc test. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001 were considered as significant. Credit: Science Advances (2023). DOI: 10.1126/sciadv.adf9284"/>